Jarred Yasuhara-Bell, Ph.D.

Jarred Yasuhara-Bell, PhD is currently a Post-Doctoral Fellow in the Department of Plant Pathology at Kansas State University, United States. Dr. Yasuhara-Bell obtained his B.S. in Bioengineering from Oregon State University. He then went back home where he attended the University of Hawaiʻi at Mānoa and went on to earn an M.S. in Tropical Medicine, Medical Microbiology and Pharmacology and a Ph.D. in Molecular Biosciences and Bioengineering. During his Master’s program, he screened marine microorganism extracts for antiviral activity against two mammalian viruses. During his Doctoral program, he worked in the renowned plant bacteriology laboratory under Dr. Anne Alvarez. His doctoral research focused on development and validation of a specific nucleic acid-based detection assay for the regulated pathogen (in the European Union) causing bacterial canker of tomato, Clavibacter michiganensis subsp. michiganensis (Cmm), using loop-mediated isothermal amplification (LAMP) technology, for practical implementation as a portable field assay and seed test. This research also involved the investigation of two distinct populations of non-Cmm Clavibacter spp. that were also isolated from tomato. While completing his dissertation research, he also undertook the lead on an interdepartmental team project to develop molecular diagnostic tools for detecting and differentiating two Klebsiella species (K. oxytoca and K. variicola), both of which have plant-associated, veterinary and clinical isolates.

He continued on as a post-doctoral researcher at the University of Hawaiʻi at Mānoa and completed work on three LAMP assays for detecting, identifying and differentiating Clavibacter michiganensis subsp. nebraskensis, Pectobacterium carotovorum and Dickeya spp. Additionally, he evaluated a recombinase-polymerase amplification (RPA) and LAMP assay for detection of Cmm and Ralstonia solanacearum race 3 biovar 2 (a select agent), respectively. The RPA assay was developed by Agdia Inc. and was based on his doctoral research. He was asked to coauthor two sections of the Laboratory Guide for Identification of Plant Pathogenic Bacteria, 4th ed., including a section on “LAMP and isothermal amplification” and an appendix chapter on “Serological Techniques.” This publication is still pending publication through the American Phytopathological Society (APS) Press. He was also given the task of developing a laboratory manual for Plant and Environmental Protection Sciences (PEPS) Biology of Plant Pathogens: Module I – Bacteria (PEPS 606) course.

Dr. Yasuhara-Bell has worked on a field-deployable LAMP-based diagnostic assay for the detection of the wheat blast pathogen Magnaporthe oryzae Triticum. Currently, Dr. Yasuhara-Bell is working in collaboration with Plant Biosecurity Cooperative Research Centre (Canberra, Australia), AgriBio Centre for AgriBioscience at La Trobe University (Melbourne, VIC, Australia), and Murdoch University (Perth, WA, Australia), on a high consequence pathogen, Rathayibacter toxicus. He is working on whole genome-based comparative genomics, population genetics, evolution, genome-informed diagnostics and CRISPR-CAS system of R. toxicus.

Publications

2018

Yasuhara-Bell J and Stack JP. 2018. Panel of three loop-mediated isothermal amplification assays allow Rathayibacter toxicus subpopulation differentiation. J. Plant Pathol. (Submitted for Review).

Yasuhara-Bell J, Pedley KF, Farman M, Valent B and Stack JP. 2018. Specific detection of the wheat blast pathogen (Magnaporthe oryzae Triticum) by loop-mediated isothermal amplification. Plant Dis. https://doi.org/10.1094/PDIS-03-18-0512-RE

2017

Yasuhara-Bell J, Marrero G, Arif M, de Silva A and Alvarez AM., 2017. Development of a loop-mediated isothermal amplification assay for the detection of Dickeya spp. Phytopathology 107(11):1339-1345.

2016

Yasuhara‐Bell J, Marrero G, de Silva A and Alvarez AM. 2016. Specific detection of Pectobacterium carotovorum by loop‐mediated isothermal amplification. Mol. Plant Pathol. 17(9)1499-1505.

Yasuhara-Bell J, de Silva A, Heuchelin SA, Chaky JL and Alvarez AM. 2016. Detection of Goss’s wilt pathogen Clavibacter michigenensis subsp. nebraskensis in maize by loop-mediated amplification. Phytopathology. 106(4)226-235.

2015

Yasuhara-Bell J, Ayin C, Hatada A, Yoo Y, Schlub RL and Alvarez AM. 2015. Specific detection of Klebsiella variicola and K. oxytoca by loop-mediated isothermal amplification. J. Plant Pathol. Microbiol. 6(5):271.

Yasuhara-Bell J, Baysal-Gurel F, Miller S and Alvarez AM. 2015. Utility of a loop-mediated amplification assay for detection of Clavibacter michiganensis subsp. michiganensis in seeds and plant tissues. Can. J. Plant Pathol. 37(3):260-266.

Yasuhara-Bell J and Alvarez AM. 2015. Seed-associated subspecies of the genus Clavibacter are clearly distinguishable from Clavibacter michiganensis subsp. michiganensis. Int. J. Syst. Evol. Microbiol. 65(3):811-826.

Ayin C, Schlub R, Yasuhara-Bell J and Alvarez AM. 2015. Identification and characterization of bacteria associated with decline of ironwood (Casuarina equisetifolia) in Guam. Australas. Plant Pathol. 44(2):225-234.

Yasuhara-Bell J and Alvarez AM. 2015. Differentiation of Clavibacter michiganensis subsp. michiganensis from other Clavibacter species found in seed and plant tissues. Acta Hort. 1069:87-94.

2014

Yasuhara-Bell J, Marrero G and Alvarez AM. 2014. Genes clvA, clvF and clvG are unique to Clavibacter michiganensis subsp. michiganensis and highly conserved. Eur. J. Plant Pathol. 140(4):655-664.

2013

Yasuhara-Bell J, Kubota R, Jenkins DM and Alvarez AM. 2013. Loop-mediated amplification of the Clavibacter michiganensis subsp. michiganensis micA gene is highly specific.  Phytopathology. 103(12):1220-1226.

Yasuhara-Bell J, de Silva A, Ko M, Shimabukuro R and Alvarez A. 2013. First report in Hawaiʻi of Xanthomonas citri pv. mangiferaeindicae causing bacterial black spot on Mangifera indica. Plant Dis. 97(9):1244.

Lab activities and updates for the J Stack Lab at Kansas State University

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